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murine insulin-like growth factor ii (migf-ii  (R&D Systems)


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    R&D Systems murine insulin-like growth factor ii (migf-ii
    Murine Insulin Like Growth Factor Ii (Migf Ii, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/migf+ii/murine+insulin+like+growth+factor+ii++migf+ii/pmc07805318-48-67-73
    Average 90 stars, based on 1 article reviews
    murine insulin-like growth factor ii (migf-ii - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Generation of a lentiviral vector producer cell clone for human Wiskott-Aldrich syndrome gene therapy
    Article Snippet: Lineage depleted (Lin – ) murine WASp – cells were obtained by harvesting bone marrow cells from tibias and femurs of WASp –/– and WASp – mice, and recovering lineage depleted cells using a commercially available kit (Miltenyi Biotech; cat. no. 130-092-211). .. The cells were collected by centrifugation at 1,500 rpm for 10 minutes at 4 °C, then resuspended in StemSpan SFEM medium (Stem Cell Technologies; Vancouver, BC, Canada; cat. no. 09650), containing 10 ng/ml mSCF (Peprotech; cat. no. 250-03), 20 ng/ml mTPO (Peprotech, NJ; cat. no. 315-14), 20 ng/ml mIGF-II (R&D Systems, Minneapolis, MN; cat. no. 792-MG), 10 ng/ml hFGF (Peprotech; cat. no. 100-17A), 10 μg/ml Heparin (Sigma, St Louis, MO; cat. no. H3149), 1×Glut, and 1× phosphate-buffered saline (PBS), henceforth designated as Lin – culture medium. .. For transduction, 7.5 μg/ml polybrene (Sigma; cat. no. H9268) was added to the Lin – culture medium.

    Saline:

    Article Title: Generation of a lentiviral vector producer cell clone for human Wiskott-Aldrich syndrome gene therapy
    Article Snippet: Lineage depleted (Lin – ) murine WASp – cells were obtained by harvesting bone marrow cells from tibias and femurs of WASp –/– and WASp – mice, and recovering lineage depleted cells using a commercially available kit (Miltenyi Biotech; cat. no. 130-092-211). .. The cells were collected by centrifugation at 1,500 rpm for 10 minutes at 4 °C, then resuspended in StemSpan SFEM medium (Stem Cell Technologies; Vancouver, BC, Canada; cat. no. 09650), containing 10 ng/ml mSCF (Peprotech; cat. no. 250-03), 20 ng/ml mTPO (Peprotech, NJ; cat. no. 315-14), 20 ng/ml mIGF-II (R&D Systems, Minneapolis, MN; cat. no. 792-MG), 10 ng/ml hFGF (Peprotech; cat. no. 100-17A), 10 μg/ml Heparin (Sigma, St Louis, MO; cat. no. H3149), 1×Glut, and 1× phosphate-buffered saline (PBS), henceforth designated as Lin – culture medium. .. For transduction, 7.5 μg/ml polybrene (Sigma; cat. no. H9268) was added to the Lin – culture medium.



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